COX6B1 Recombinant Rabbit Monoclonal Antibody [JE59-16]
cat.: HA722407
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE59-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human COX6B1 aa 37-86 / 86. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, Caco-2 cell lysate, human colon tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:8,000 1:1,000-1:4,000 |
| Uniprot #: | SwissProt: P14854 Human |
| Alternative names: | COX 6B COX VIb 1 COX VIb-1 COX6B COX6B1 COXG COXVIb1 CX6B1_HUMAN Cytochrome c oxidase subunit 6B1 Cytochrome c oxidase subunit VIb Cytochrome c oxidase subunit VIb isoform 1 cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) Cytochrome c oxidase subunit Vib polypeptide 1 |
Images
|
Fig1:
Western blot analysis of COX6B1 on different lysates with Rabbit anti-COX6B1 antibody (HA722407) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722407) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-COX6B1 antibody (HA722407) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722407) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.