MSRB3 Recombinant Rabbit Monoclonal Antibody [JE64-83]
cat.: HA722410
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-83 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MSRB3 aa 1-200 / 192. |
| Positive control: | A549 cell lysate, HUVEC cell lysate, U-2 OS cell lysate, HeLa cell lysate, mouse testis tissue lysate, mouse heart tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Endoplasmic reticulum, Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q8IXL7 Human | Q8BU85 Mouse Entrez Gene: 680036 Rat |
| Alternative names: | Deafness, Autosomal Recessive 74 DFNB74 FLJ36866 Methionine R sulfoxide reductase B mitochondrial Methionine sulfoxide reductase B3 Methionine-R-sulfoxide reductase B3 MsrB3 MSRB3_HUMAN |
Images
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Fig1:
Western blot analysis of MSRB3 on different lysates with Rabbit anti-MSRB3 antibody (HA722410) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HUVEC cell lysate (20 µg/Lane) Lane 3: U-2 OS cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: Mouse testis tissue lysate (30 µg/Lane) Lane 6: Mouse heart tissue lysate (30 µg/Lane) Lane 7: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722410) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.