PRD Recombinant Rabbit Monoclonal Antibody [JE65-13]
cat.: HA722411
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-13 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PRD aa 444-493 / 493. |
| Positive control: | SK-Br-3 cell lysate, HEK-293 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate. |
| Subcellular location: | Extracellular exosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P12955 Human |
| Alternative names: | Aminoacyl L proline hydrolase Imidodipeptidase MGC10905 MGC95081 Pep 4 Pep4 pepD PEPD_HUMAN Peptidase 4 Peptidase D Prolidase Proline dipeptidase X pro dipeptidase X-Pro dipeptidase Xaa-Pro dipeptidase |
Images
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Fig1:
Western blot analysis of PRD on different lysates with Rabbit anti-PRD antibody (HA722411) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: SH-SY5Y cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722411) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.