PWP1 Recombinant Rabbit Monoclonal Antibody [JE65-28]
cat.: HA722412
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PWP1 aa 452-501 / 501. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, C6 cell lysate. |
| Subcellular location: | Nucleus, nucleolus, Chromosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q13610 Human | Q99LL5 Mouse Entrez Gene: 362856 Rat |
| Alternative names: | Keratinocyte protein IEF SSP 9502 Periodic tryptophan protein 1 homolog PWP1 PWP1 homolog PWP1_HUMAN |
Images
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Fig1:
Western blot analysis of PWP1 on different lysates with Rabbit anti-PWP1 antibody (HA722412) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: Lane 1-2: 25 seconds; Lane 3-4: 1 minute 6 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722412) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.