ABCD1 / ALD Recombinant Rabbit Monoclonal Antibody [JE75-07]
cat.: HA722413
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE75-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ABCD1 / ALD aa 596-745 / 745. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, 293T cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, RAW264.7, C6. |
| Subcellular location: | Peroxisome membrane, Mitochondrion membrane, Lysosome membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P33897 Human |
| Alternative names: | ABC42 Abcd1 ABCD1_HUMAN Adrenoleukodystrophy protein ALD Aldgh ALDP AMN ATP binding cassette, sub family D (ALD), member 1 ATP-binding cassette sub-family D member 1 OTTHUMP00000025960 OTTMUSP00000019283 RGD1562128 RP23 373N8.2 X linked adrenoleukodystrophy (ALD) gene homolog |
Images
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Fig1:
Western blot analysis of ABCD1 / ALD on different lysates with Rabbit anti-ABCD1 / ALD antibody (HA722413) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: 293T cell lysate Lane 4: RAW264.7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 75 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722413) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling ABCD1 / ALD with Rabbit anti-ABCD1 / ALD antibody (HA722413) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ABCD1 / ALD antibody (HA722413) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunocytochemistry analysis of RAW264.7 cells labeling ABCD1 / ALD with Rabbit anti-ABCD1 / ALD antibody (HA722413) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ABCD1 / ALD antibody (HA722413) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig4:
Immunocytochemistry analysis of C6 cells labeling ABCD1 / ALD with Rabbit anti-ABCD1 / ALD antibody (HA722413) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ABCD1 / ALD antibody (HA722413) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.