ACADVL / VLCAD Recombinant Rabbit Monoclonal Antibody [JE77-50]
cat.: HA722416
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JE77-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ACADVL / VLCAD aa 1-100 / 655. |
| Positive control: | HEK-293 cell lysate, A431 cell lysate, HepG2 cell lysate, A431, human small intestine tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell IF-Tissue IP |
1:1,000 1:1,000 1:100 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: P49748 Human |
| Alternative names: | ACAD 6 ACAD6 ACADV_HUMAN Acadvl Acyl CoA dehydrogenase very long chain Acyl Coenzyme A dehydrogenase very long chain LCACD mitochondrial Very long chain specific acyl CoA dehydrogenase Very long chain specific acyl CoA dehydrogenase mitochondrial Very long-chain specific acyl-CoA dehydrogenase VLCAD |
Images
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Fig1:
Western blot analysis of ACADVL / VLCAD on different lysates with Rabbit anti-ACADVL / VLCAD antibody (HA722416) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A431 cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722416) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling ACADVL / VLCAD with Rabbit anti-ACADVL / VLCAD antibody (HA722416) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ACADVL / VLCAD antibody (HA722416) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ACADVL / VLCAD antibody (HA722416) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722416) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
ACADVL / VLCAD was immunoprecipitated from 0.2 mg A431 cell lysate with HA722416 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722416 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA722416 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA722416 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 47 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.