EDG3 / S1P3 Recombinant Rabbit Monoclonal Antibody [JE53-79]
cat.: HA722419
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE53-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human EDG3/S1P3 aa 30-150. |
| Positive control: | HepG2 cell lysate, 293T cell lysate, PC-12 cell lysate, human heart tissue, human kidney tissue, mouse heart tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q99500 Human | Q9Z0U9 Mouse Entrez Gene: 306792 Rat |
| Alternative names: | EDG 3 EDG3 Endothelial differentiation G protein coupled receptor 3 Endothelial differentiation G-protein coupled receptor 3 Endothelial differentiation gene 3 Endothelial differentiation sphingolipid G protein coupled receptor 3 FLJ37523 FLJ93220 G protein coupled receptor endothelial differentiation gene 3 LPB 3 LPB3 MGC71696 OTTHUMP00000215691 S1P receptor 3 S1P receptor Edg 3 S1P receptor Edg-3 S1P receptor EDG3 S1P3 S1pr3 S1PR3_HUMAN Sphingosine 1 phosphate receptor 3 Sphingosine 1 phosphate receptor Edg 3 Sphingosine 1 phosphate receptor Edg3 Sphingosine 1-phosphate receptor 3 Sphingosine 1-phosphate receptor Edg-3 |
Images
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Fig1:
Western blot analysis of EDG3 / S1P3 on different lysates with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/1,000 dilution. Lane 1: HepG2 cell lysate, 20 µg/Lane Lane 2: 293T cell lysate, 20 µg/Lane Lane 3: PC-12 cell lysate, 20 µg/Lane Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722419) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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