MUSK Recombinant Rabbit Monoclonal Antibody [PSH05-68]
cat.: HA722420
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH05-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MUSK aa 1-516 / 869. |
| Positive control: | Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue. |
| Subcellular location: | Postsynaptic cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000 1:2,000 1:100 |
| Uniprot #: | SwissProt: O15146 Human | Q61006 Mouse | Q62838 Rat |
| Alternative names: | CMS9 FADS MDK 4 MDK4 MGC126323 MGC126324 Muscle Muscle associated receptor tyrosine kinase Muscle skeletal receptor tyrosine kinase Muscle skeletal receptor tyrosine protein kinase Muscle specific kinase receptor Muscle specific tyrosine kinase receptor Muscle specific tyrosine protein kinase receptor Muscle-specific kinase receptor Muscle-specific tyrosine-protein kinase receptor MuSK MUSK_HUMAN Neural fold somite kinase 1 Neural fold somite kinase 2 Neural fold somite kinase 3 Neural fold somite kinase1 Neural fold somite kinase2 Neural fold somite kinase3 Nsk 1 Nsk 2 Nsk 3 Nsk1 Nsk2 Nsk3 Receptor tyrosine kinase MuSK Skeletal muscle receptor tyrosine kinase skeletal receptor tyrosine-protein kinase |
Images
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Fig1:
Western blot analysis of MUSK on different lysates with Rabbit anti-MUSK antibody (HA722420) at 1/2,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate (no heat) Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722420) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-MUSK antibody (HA722420) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722420) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-MUSK antibody (HA722420) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722420) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MUSK antibody (HA722420) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722420) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded human skeletal muscle tissue labeling MUSK with Rabbit anti-MUSK antibody (HA722420) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722420, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.