Rat IL-2 Recombinant Rabbit Monoclonal Antibody [PSH05-71]
cat.: HA722423
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Rat
Applications: ELISA(Cap)
Clonality: Monoclonal
Clone number: PSH05-71
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 17.6 kDa
Isotype: IgG
Immunogen: Recombinant protein within Rat IL-2 aa 21-155.
Positive control: Recombinant standard Rat IL-2 protein (HA210683).
Recommended Dilutions:
  ELISA(Cap)

Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-72] to Rat IL-2 (Detecor) (HA722424) and recombinant standard Rat IL-2 (HA210683) as the standard. The reference range value is 30.9-2500 pg/ml.
Uniprot #: SwissProt: P17108 Rat
Alternative names: Aldesleukin IL 2 IL-2 IL2 IL2_HUMAN Interleukin 2 Interleukin-2 interleukin2 Involved in regulation of T cell clonal expansion Lymphokine OTTHUMP00000164090 POIL2 T Cell Growth Factor T-cell growth factor TCGF
Images
HA722423_1.png Fig1: Sandwich ELISA analysis of rat IL-2 matched pair antibodies

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722423) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted recombinant standard Rat IL-2 protein (HA210683) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722423, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA722423_2.png Fig2: Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen(18 hours) supernatant samples.

Rat spleen was cultured for 18 hours in the presence or absence of 3 µg/ml Concanavalin A. The concentrations of IL-2 measured in duplicate and interpolated from the IL-2 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 8% and stimulated 8%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 4,659 pg/ml in Concanavalin A stimulated rat spleen supernatant and undetectable in unstimulated rat spleen supernatant.
HA722423_3.png Fig3: Interpolated concentrations of spiked IL-2 in rat cell culture media samples.

The concentrations of IL-2 were interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.