TBPL1 Recombinant Rabbit Monoclonal Antibody [JE30-79]
cat.: HA722426
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JE30-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TBPL1 aa 87-186 / 186. |
| Positive control: | HeLa cell lysate, 293T cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue, 293T. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue FC IP |
1:1,000 1:1,000 1:200 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P62380 Human |
| Alternative names: | TATA box-binding protein-like 1 TBP-like 1 21 kDa TBP-like protein Second TBP of unique DNA protein (STUD) TATA box-binding protein-related factor 2 (TBP-related factor 2) TBP-like factor TBP-related protein TBPL1 TLF TLP TLP21 TRF2 TRP |
Images
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Fig1:
Western blot analysis of TBPL1 on different lysates with Rabbit anti-TBPL1 antibody (HA722426) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (40 µg/Lane) Lane 4: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 21 kDa Observed band size: 25 kDa Exposure time: 20 second; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722426) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TBPL1 antibody (HA722426) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722426) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TBPL1 antibody (HA722426) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722426) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TBPL1 antibody (HA722426) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722426) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of 293T cells labeling TBPL1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722426, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
TBPL1 was immunoprecipitated from 0.2 mg 293T cell lysate with HA722426 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722426 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/50,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cell lysate (input) Lane 2: HA722426 IP in 293T cell lysate Lane 3: Rabbit IgG instead of HA722426 in 293T cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 14 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.