Gemin 2 Recombinant Rabbit Monoclonal Antibody [JE33-32]
cat.: HA722428
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC, IP
Clonality: Monoclonal
Clone number: JE33-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 32 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Gemin 2 aa 1-100.
Positive control: HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HeLa.
Subcellular location: Nucleus, gem, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  FC
  IP

1:1,000
1:100
1:1,000
1-2μg/sample
Uniprot #: SwissProt: O14893 Human | Q9CQQ4 Mouse | Q9QZP1 Rat
Alternative names: Component of gems 2 Gem (nuclear organelle) associated protein 2 Gem associated protein 2 GEMI2_HUMAN Gemin-2 gemin2 SIP 1 SIP-1 SIP1 SIP1 delta SIP1-delta SMN interacting protein 1 SMN interacting protein 1 delta SMN-interacting protein 1 Survival interacting protein 1 Survival of motor neuron protein interacting protein 1 Survival of motor neuron protein-interacting protein 1
Images
HA722428_1.jpg Fig1: Western blot analysis of Gemin 2 on different lysates with Rabbit anti-Gemin 2 antibody (HA722428) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)
Lane 5: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 32 kDa
Observed band size: 32 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722428) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722428_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling Gemin 2 with Rabbit anti-Gemin 2 antibody (HA722428) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Gemin 2 antibody (HA722428) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722428_3.jpg Fig3: Flow cytometric analysis of HeLa cells labeling Gemin 2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722428, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA722428_4.jpg Fig4: Gemin 2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722428 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722428 at 1/1,000 dilution. Alpaca anti-Rabbit IgG Fc, Recombinant VHH secondary antibody (HA1031) at 1/50,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: HA722428 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722428 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 2 minutes 29 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.