MRC2 Recombinant Rabbit Monoclonal Antibody [JE34-76]
cat.: HA722429
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE34-76
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 167 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human MRC2 aa 101-250 / 1,479.
Positive control: HEK-293 cell lysate, Saos-2 cell lysate.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9UBG0 Human
Alternative names: C-type lectin domain family 13 member E C-type mannose receptor 2 CD 280 CD280 CD280 antigen CLEC 13E CLEC13E ENDO 180 ENDO180 Endocytic receptor (macrophage mannose receptor family) Endocytic receptor 180 FLJ35911 KIAA0709 Macrophage mannose receptor 2 Mannose receptor C type 2 MRC 2 MRC2 MRC2_HUMAN UPAR-associated protein UPARAP Urokinase plasminogen activator receptor associated protein Urokinase receptor associated protein Urokinase receptor-associated protein Urokinase-type plasminogen activator receptor-associated protein
Images
HA722429_1.jpg Fig1: Western blot analysis of MRC2 on different lysates with Rabbit anti-MRC2 antibody (HA722429) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HEK-293 cell lysate treated with deglycosylation
Lane 3: Saos-2 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 167 kDa
Observed band size: 167-250 kDa

Exposure time: Lane 1-2: 3 minutes; Lane 3: 4 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722429) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.