| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE34-76 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 167 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MRC2 aa 101-250 / 1,479. |
| Positive control: | HEK-293 cell lysate, Saos-2 cell lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9UBG0 Human |
| Alternative names: | C-type lectin domain family 13 member E C-type mannose receptor 2 CD 280 CD280 CD280 antigen CLEC 13E CLEC13E ENDO 180 ENDO180 Endocytic receptor (macrophage mannose receptor family) Endocytic receptor 180 FLJ35911 KIAA0709 Macrophage mannose receptor 2 Mannose receptor C type 2 MRC 2 MRC2 MRC2_HUMAN UPAR-associated protein UPARAP Urokinase plasminogen activator receptor associated protein Urokinase receptor associated protein Urokinase receptor-associated protein Urokinase-type plasminogen activator receptor-associated protein |
|
Fig1:
Western blot analysis of MRC2 on different lysates with Rabbit anti-MRC2 antibody (HA722429) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 cell lysate treated with deglycosylation Lane 3: Saos-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 167 kDa Observed band size: 167-250 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3: 4 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722429) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |