APPL Recombinant Rabbit Monoclonal Antibody [JE35-02]
cat.: HA722430
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE35-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human APPL aa 400-500. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat lung tissue lysate, human pancreas tissue, mouse pancreas tissue, rat pancreas tissue. |
| Subcellular location: | Early endosome membrane, Nucleus, Cytoplasm, Endosome, Cell projection, ruffle, Cytoplasmic vesicle, phagosome. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: Q9UKG1 Human |
| Alternative names: | Adapter protein containing PH domain Adaptor Protein Containing pH Domain Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 Adaptor protein containing pH domain PTB domain and leucine zipper motif Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 AKT2 INTERACTOR APPL 1 Appl1 DCC interacting protein 13 alpha DCC-interacting protein 13-alpha DIP 13 alpha DIP13 alpha Dip13-alpha DIP13alpha DP13A_HUMAN KIAA1428 PTB domain and leucine zipper motif 1 PTB Domain and Leucine Zipper Motif Signaling adaptor protein DIP13alpha |
Images
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Fig1:
Western blot analysis of APPL on different lysates with Rabbit anti-APPL antibody (HA722430) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722430) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-APPL antibody (HA722430) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722430) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-APPL antibody (HA722430) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722430) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-APPL antibody (HA722430) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722430) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
APPL was immunoprecipitated from 0.2 mg HEK-293 cell lysate with HA722430 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722430 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEK-293 cell lysate (input) Lane 2: HA722430 IP in HEK-293 cell lysate Lane 3: Rabbit IgG instead of HA722430 in HEK-293 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.