Tin2 Recombinant Rabbit Monoclonal Antibody [JE35-82]
cat.: HA722432
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-82 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Tin2 aa 151-199 / 451. |
| Positive control: | MCF7 cell lysate, HUVEC cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Nucleus, Chromosome, telomere; Nucleus matrix. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9BSI4 Human | Q9QXG9 Mouse Entrez Gene: 290232 Rat |
| Alternative names: | AW552114 D14Wsu146e DKCA3 MGC94711 TERF 1 (TRF 1) interacting nuclear factor 2 TERF 1 interacting nuclear factor 2 TERF1 (TRF1) interacting nuclear factor 2 TERF1 interacting nuclear factor 2 TERF1-interacting nuclear factor 2 Tin 2 TIN2 TINF 2 Tinf2 TINF2_HUMAN TRF 1 interacting nuclear factor 2 TRF1 interacting nuclear factor 2 TRF1-interacting nuclear protein 2 |
Images
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Fig1:
Western blot analysis of Tin2 on different lysates with Rabbit anti-Tin2 antibody (HA722432) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HUVEC cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 45 kDa Exposure time: 1 minute 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722432) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.