Tin2 Recombinant Rabbit Monoclonal Antibody [JE35-82]
cat.: HA722432
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE35-82
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Tin2 aa 151-199 / 451.
Positive control: MCF7 cell lysate, HUVEC cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate.
Subcellular location: Nucleus, Chromosome, telomere; Nucleus matrix.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9BSI4 Human | Q9QXG9 Mouse
Entrez Gene: 290232 Rat
Alternative names: AW552114 D14Wsu146e DKCA3 MGC94711 TERF 1 (TRF 1) interacting nuclear factor 2 TERF 1 interacting nuclear factor 2 TERF1 (TRF1) interacting nuclear factor 2 TERF1 interacting nuclear factor 2 TERF1-interacting nuclear factor 2 Tin 2 TIN2 TINF 2 Tinf2 TINF2_HUMAN TRF 1 interacting nuclear factor 2 TRF1 interacting nuclear factor 2 TRF1-interacting nuclear protein 2
Images
HA722432_1.jpg Fig1: Western blot analysis of Tin2 on different lysates with Rabbit anti-Tin2 antibody (HA722432) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: HUVEC cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 45 kDa

Exposure time: 1 minute 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722432) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.