PPCS Recombinant Rabbit Monoclonal Antibody [JE63-91]
cat.: HA722435
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JE63-91 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PPCS aa 262-311 / 311. |
| Positive control: | HeLa cell lysate, 293T cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, A431 cell lysate, human colon tissue, human colon cancer tissue, human kidney tissue, A431. |
| Subcellular location: | Cytoplasm, cytosol, nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue FC IP |
1:1,000 1:200-1:1,000 1:200 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9HAB8 Human |
| Alternative names: | COAB FLJ11838 MGC117357 MGC138220 OTTHUMP00000008433 Phosphopantothenate--cysteine ligase Phosphopantothenoylcysteine synthetase PPC synthetase Ppcs PPCS_HUMAN RP11-163G10.1 |
Images
|
Fig1:
Western blot analysis of PPCS on different lysates with Rabbit anti-PPCS antibody (HA722435) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722435) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PPCS antibody (HA722435) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PPCS antibody (HA722435) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722435) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PPCS antibody (HA722435) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Flow cytometric analysis of A431 cells labeling PPCS. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722435, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig6:
PPCS was immunoprecipitated from 0.2 mg 293T cell lysate with HA722435 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722435 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cell lysate (input) Lane 2: HA722435 IP in 293T cell lysate Lane 3: Rabbit IgG instead of HA722435 in 293T cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 20 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.