NDUFA1 Recombinant Rabbit Monoclonal Antibody [JE64-37]
cat.: HA722436
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 8 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NDUFA1 aa 20-70. |
| Positive control: | K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, 293T cell lysate, human heart tissue, human skeletal muscle tissue, human kidney tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: O15239 Human |
| Alternative names: | CI MWFE CI-MWFE Complex I MWFE complex I MWFE subunit Complex I-MWFE MWFE MWFE, B. taurus, homolog of NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 1 7.5kDa NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 NADH oxidoreductase subunit MWFE NADH ubiquinone oxidoreductase (complex 1) NADH ubiquinone oxidoreductase MWFE subunit NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 1 NADH-ubiquinone oxidoreductase MWFE subunit NDUA1_HUMAN NDUFA 1 NDUFA1 Type I dehydrogenase ZNF 183 ZNF183 |
Images
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Fig1:
Western blot analysis of NDUFA1 on different lysates with Rabbit anti-NDUFA1 antibody (HA722436) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: 293T cell lysate (20 µg/Lane) Predicted band size: 8 kDa Observed band size: 10 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722436) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-NDUFA1 antibody (HA722436) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722436) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-NDUFA1 antibody (HA722436) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722436) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFA1 antibody (HA722436) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722436) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.