Pyrophosphatase 1 Recombinant Rabbit Monoclonal Antibody [JE64-39]
cat.: HA722437
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE64-39
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 33 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Pyrophosphatase 1 aa 1-100.
Positive control: HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q15181 Human | Q9D819 Mouse
Entrez Gene: 294504 Rat
Alternative names: cytosolic inorganic pyrophosphatase diphosphate phosphohydrolase inorganic diphosphatase inorganic pyrophosphatase 1 Inorganic pyrophosphatase IOPPP IPYR_HUMAN PP PP1 PPA1 PPase Pyp pyrophosphatase, inorganic, 1 Pyrophosphate phospho hydrolase Pyrophosphate phospho-hydrolase SID6-8061
Images
HA722437_1.jpg Fig1: Western blot analysis of Pyrophosphatase 1 on different lysates with Rabbit anti-Pyrophosphatase 1 antibody (HA722437) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A431 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722437) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.