Delta-catenin Recombinant Rabbit Monoclonal Antibody [JE75-34]
cat.: HA722438
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JE75-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 135 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Catenin delta-2 aa 1-100 / 1,247. |
| Positive control: | Mouse brain tissue lysate, mouse hippocampus tissue lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, rat cerebellum tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Nucleus, Cell junction, adherens junction, Cell projection, dendrite, Perikaryon. |
| Recommended Dilutions:
WB IHC-P IP IHC-Fr |
1:1,000 1:200 1-2μg/sample 1:200 |
| Uniprot #: | SwissProt: Q9UQB3 Human | O35927 Mouse | O35116 Rat |
| Alternative names: | ARM-repeat protein Catenin (cadherin associated protein) delta 2 catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein) Catenin delta 2 Catenin delta-2 CTND2_HUMAN CTNND 2 Ctnnd2 Delta catenin Delta-catenin GT 24 GT24 Neural plakophilin related arm repeat protein Neural plakophilin related armadillo repeat protein Neural plakophilin-related ARM-repeat protein Neural plakophilin-related, Neurojungin Neurojungin NPRAP T cell delta catenin |
Images
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Fig1:
Western blot analysis of Delta-catenin on different lysates with Rabbit anti-Delta-catenin antibody (HA722438) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Mouse heart tissue lysate (negative) Lane 4: Mouse kidney tissue lysate (negative) Lane 5: Mouse cerebellum tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat heart tissue lysate (negative) Lane 8: Rat kidney tissue lysate (negative) Lane 9: Rat spleen tissue lysate (negative) Lane 10: Rat hippocampus tissue lysate Lane 11: Rat cerebellum tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 135 kDa Observed band size: 135 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722438) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Delta-catenin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722438 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722438 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA722438 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA722438 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds; ECL: K1801 |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722438) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-Delta-catenin antibody (HA722438) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722438, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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