Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) Recombinant Rabbit Monoclonal Antibody [PSH05-73]
cat.: HA722440
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH05-73 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein. |
| Positive control: | HeLa starved for 3 hours then add 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Cell membrane. Cell projection. Cytoplasm. Cytoskeleton. Membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P15311 Human | P26038 Human | P35241 Human | P26040 Mouse | P26041 Mouse | P26043 Mouse | P31977 Rat | O35763 Rat |
| Alternative names: | Villin 2 ezrin CVIL CVL Cytovillin 2 Cytovillin DKFZp762H157 Epididymis secretory protein Li 105 EZR EZRI_HUMAN Ezrin FLJ26216 HEL S 105 MGC1584 p81 VIL 2 VIL2 Villin 2 (ezrin) Villin 2 Villin-2 Villin2 CB567 CG12537 DFNB24 ESP10 Hh-induced MATH and BTB domain-containing protein HIB Moesin-B Protein roadkill RADI_HUMAN Radixin RDX Epididymis luminal protein 70 HEL70 Membrane organizing extension spike protein Membrane-organizing extension spike protein MOES_HUMAN Moesin Moesin/anaplastic lymphoma kinase fusion protein Msn MSN/ALK fusion |
Images
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Fig1:
Western blot analysis of Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) on different lysates with Rabbit anti-Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) antibody (HA722440) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa starved for 3 hours cell lysate Lane 2: HeLa starved for 3 hours then add 100nM Calyculin A for 30 minutes cell lysate Lane 3: NIH/3T3 starved for 24 hours cell lysate Lane 4: NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 75/80 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722440) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue untreated / treated with λpp with Rabbit anti-Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) antibody (HA722440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat liver tissue untreated / treated with λpp with Rabbit anti-Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) antibody (HA722440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells untreated (upper, positive) and HeLa cells treated with 1μM staurosporine for 3 hours (lower, negative) labeling Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) with Rabbit anti-Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) antibody (HA722440) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Ezrin (T567)/Radixin (T564)/Moesin (T558) antibody (HA722440) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.