Steroid sulfatase Recombinant Rabbit Monoclonal Antibody [PSH05-74]
cat.: HA722441
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH05-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Steroid sulfatase aa 22-184 and aa 235-583. |
| Positive control: | Jurkat cell lysate HepG2 cell lysate, MCF7 cell lysate, Huh7 cell lysate, T-47D cell lysate, human placenta tissue. |
| Subcellular location: | Cytoplasmic vesicle. Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:3,000 |
| Uniprot #: | SwissProt: P08842 Human |
| Alternative names: | ARSC ARSC1 Arylsulfatase C Arylsulfatase C isozyme S ASC EC 3.1.6.2 ES Estrone sulfatase SSDD Steroid sulfatase (microsomal) Steroid sulfatase (microsomal) arylsulfatase C isozyme S Steroid sulfatase Steryl sulfatase Steryl sulfatase precursor Steryl sulfate sulfohydrolase Steryl-sulfatase Steryl-sulfate sulfohydrolase STS STS_HUMAN |
Images
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Fig1:
Western blot analysis of Steroid sulfatase on different lysates with Rabbit anti-Steroid sulfatase antibody (HA722441) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: LNCaP cell lysate (negative) Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: Huh7 cell lysate Lane 6: T-47D cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722441) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Steroid sulfatase antibody (HA722441) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722441) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Steroid sulfatase antibody (HA722441) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722441) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.