MFGE8 Recombinant Rabbit Monoclonal Antibody [PSH05-75]
cat.: HA722442
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH05-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MFGE8 aa 1-387. |
| Positive control: | A549 cell lysate, A431 cell lysate, HeLa cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, BT-20 cell lysate. |
| Subcellular location: | Membrane, Secreted, Cytoplasmic vesicle, secretory vesicle, acrosome membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q08431 Human | P21956 Mouse | P70490 Rat |
| Alternative names: | BA46 Breast epithelial antigen BA46 EDIL1 HMFG hP47 HsT19888 Lactadherin Medin MFG-E8 MFGE8 MFGM MFGM_HUMAN Milk fat globule EGF factor 8 Milk fat globule EGF factor 8 protein Milk fat globule-EGF factor 8 O acetyl disialoganglioside synthase OAcGD3S SED1 SPAG10 Sperm associated antigen 10 Sperm surface protein hP47 |
Images
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Fig1:
Western blot analysis of MFGE8 on different lysates with Rabbit anti-MFGE8 antibody (HA722442) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A431 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: BT-20 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722442) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.