| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE00-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCR9 aa 1-100 / 369. |
| Positive control: | A549 cell lysate, Jurkat cell lysate, HepG2 cell lysate, mouse thymus tissue lysate, rat lung tissue lysate, rat thymus tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P51686 Human | Q9WUT7 Mouse Entrez Gene: 282832 Rat |
| Alternative names: | C C chemokine receptor D6 C C chemokine receptor type 9 C C CKR 9 C-C chemokine receptor type 9 C-C CKR-9 CC Chemokine receptor CCR10 CC CKR 9 CC-CKR-9 CCBP2 CCCKR9 CCR 9 CCR-9 Ccr9 CCR9_HUMAN CDw199 CDw199 antigen Chemokine (C C motif) receptor 9 Chemokine (C C motif) receptor 9, isoform CRA_a Chemokine (C-C motif) receptor 9 isoform A Chemokine (C-C motif) receptor 9 isoform B Chemokine C C motif receptor 9 Chemokine C C receptor 10 Chemokine CC Motif Receptor 9 Chemokine receptor CCR 9 Chemokine receptor CCR9 Chemokine-binding protein D6 Cmkbr10 CMKBR9 G protein coupled receptor 28 G-protein coupled receptor 28 GPR 28 GPR 9 6 GPR-9-6 GPR28 GPR96 OTTHUMP00000164653 OTTHUMP00000164654 |
|
Fig1:
Western blot analysis of CCR9 on different lysates with Rabbit anti-CCR9 antibody (HA722444) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: Mouse thymus tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute 50 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722444) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |