INPP4B Recombinant Rabbit Monoclonal Antibody [JE07-42]
cat.: HA722445
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE07-42 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human INPP4B aa 1-100. |
| Positive control: | HeLa cell lysate, mouse heart tissue lysate, mouse skeletal muscle tissue lysate, rat heart tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: O15327 Human | Q6P1Y8 Mouse | Q9QWG5 Rat |
| Alternative names: | Inositol polyphosphate 4 phosphatase, type II 4-bisphosphate 4-phosphatase Inositol polyphosphate 4 phosphatase type II 105kDa Inositol polyphosphate 4-phosphatase type II INP4B_HUMAN Inpp4b MGC132014 Type II inositol 3,4 bisphosphate 4 phosphatase Type II inositol-3 |
Images
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Fig1:
Western blot analysis of INPP4B on different lysates with Rabbit anti-INPP4B antibody (HA722445) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (40 µg/Lane) Lane 3: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 4: Rat heart tissue lysate (40 µg/Lane) Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: Lane 1: 30 seconds; Lane 2-5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722445) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-INPP4B antibody (HA722445) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.