IL-1RA Recombinant Rabbit Monoclonal Antibody [JE07-58]
cat.: HA722446
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE07-58 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-1RA aa 78-177 / 177. |
| Positive control: | A431 cell lysate, SW480 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Mouse lung tissue lysate, C6 cell lysate, Rat lung tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, Rat spleen tissue lysate. |
| Subcellular location: | Cytoplasm. Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P18510 Human | P25085 Mouse | P25086 Rat |
| Alternative names: | DIRA F630041P17Rik ICIL 1RA ICIL-1RA ICIL1RA IL-1ra IL-1ra3 IL-1RN IL1 inhibitor IL1F3 IL1RA IL1RA_HUMAN IL1RN (IL1F3) IL1RN Interleukin 1 receptor antagonist Interleukin-1 receptor antagonist protein Intracellular IL 1 receptor antagonist type II Intracellular interleukin 1 receptor antagonist (icIL 1ra) IRAP MGC10430 MVCD4 Type II interleukin 1 receptor antagonist |
Images
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Fig1:
Western blot analysis of IL-1RA on different lysates with Rabbit anti-IL-1RA antibody (HA722446) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: SW480 cell lysate (20 µg/Lane) Lane 3: Neuro-2a cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C2C12 cell lysate (20 µg/Lane) Lane 6: Mouse lung tissue lysate (40 µg/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722446) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of IL-1RA on different lysates with Rabbit anti-IL-1RA antibody (HA722446) at 1/1,000 dilution. Lane 1: C2C12 cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Lane 5: Rat kidney tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722446) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.