VPS11 Recombinant Rabbit Monoclonal Antibody [JE33-36]
cat.: HA722447
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-36 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 108 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human VPS11 aa aa10-44 / 941. |
| Positive control: | HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate. |
| Subcellular location: | Cytoplasmic vesicle. Endosome. Lysosome. Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9H270 Human |
| Alternative names: | END1 HGNC:14583 hVPS11 PEP5 PP3476 RING finger protein 108 RNF108 Vacuolar protein sorting 11 (yeast homolog) Vacuolar protein sorting 11 (yeast) Vacuolar protein sorting 11 homolog (S. cerevisiae) Vacuolar protein sorting 11 homolog Vacuolar protein sorting associated protein 11 homolog Vacuolar protein sorting protein 11 Vacuolar protein sorting-associated protein 11 homolog vps11 VPS11_HUMAN |
Images
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Fig1:
Western blot analysis of VPS11 on different lysates with Rabbit anti-VPS11 antibody (HA722447) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 108 kDa Observed band size: 100 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722447) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.