RPB3 Recombinant Rabbit Monoclonal Antibody [JE34-81]
cat.: HA722448
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE34-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RPB3 aa 1-150 / 275. |
| Positive control: | SiHa cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P19387 Human | P97760 Mouse Entrez Gene: 361365 Rat |
| Alternative names: | DNA directed RNA polymerase II 33 kDa polypeptide DNA directed RNA polymerase II subunit C DNA directed RNA polymerase II subunit RPB3 DNA-directed RNA polymerase II 33 kDa polypeptide DNA-directed RNA polymerase II subunit C DNA-directed RNA polymerase II subunit RPB3 hRPB33 hsRPB3 POLR 2C POLR2 C Polr2c Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa polymerase RNA II DNA directed polypeptide C 33kDa RNA polymerase II polypeptide C RNA polymerase II subunit 3 RNA polymerase II subunit B3 RPB 3 RPB3 RPB3_HUMAN RPB31 RPB33 |
Images
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Fig1:
Western blot analysis of RPB3 on different lysates with Rabbit anti-RPB3 antibody (HA722448) at 1/1,000 dilution. Lane 1: SiHa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: COS-1 cell lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (40 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722448) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.