p40/RABEPK Recombinant Rabbit Monoclonal Antibody [JE35-06]
cat.: HA722449
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human p40/RABEPK aa 223-372 / 372. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, U-87 MG cell lysate, A549 cell lysate. |
| Subcellular location: | Cytoplasm. Endosome membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q7Z6M1 Human |
| Alternative names: | 40 kDa Rab9 effector protein 8430412M01Rik 9530020d24rik AV073337 bA65N13.1 C87311 DKFZp686P1077 OTTHUMP00000022126 OTTMUSP00000012898 OTTMUSP00000012899 OTTMUSP00000041318 p40 RAB9 effector p40 Rab9 effector protein with Kelch motifs RAB9p40 RABEK_HUMAN RABEPK RABEPK protein RGD1310612 RP11 65N13.1 RP23-446N16.2 |
Images
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Fig1:
Western blot analysis of p40/RABEPK on different lysates with Rabbit anti-p40/RABEPK antibody (HA722449) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722449) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.