Smg1 Recombinant Rabbit Monoclonal Antibody [JE38-38]
cat.: HA722452
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE38-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 410 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Smg1 aa 3,562-3,661 / 3,661. |
| Positive control: | HEK-293 cell lysate, MCF7 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q96Q15 Human | Q8BKX6 Mouse Entrez Gene: 691397 Rat |
| Alternative names: | 61E3.4 ATX hSMG-1 hSMG1 KIAA0421 Lambda interacting protein Lambda-interacting protein Lambda/iota protein kinase C interacting protein Lambda/iota protein kinase C-interacting protein LIP Phosphatidylinositol 3 kinase related kinase Phosphatidylinositol 3 kinase related protein kinase PI 3 kinase related kinase SMG 1 Serine/threonine protein kinase SMG1 Serine/threonine-protein kinase SMG1 SMG-1 smg1 SMG1_HUMAN |
Images
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Fig1:
Western blot analysis of Smg1 on different lysates with Rabbit anti-Smg1 antibody (HA722452) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 410 kDa Observed band size: 410 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722452) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.