Crk p38 Recombinant Rabbit Monoclonal Antibody [JE38-60]
cat.: HA722453
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE38-60 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Crk p38 aa 1-100 / 304. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, C6 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cell membrane. Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P46108 Human | Q63768 Rat |
| Alternative names: | Adapter molecule crk Avian sarcoma virus CT10 (v crk) oncogene homolog CRK CRK isoform 2 CRK isoform II CRK_HUMAN CRKII FLJ38130 OTTHUMP00000115366 OTTHUMP00000198330 p38 Proto oncogene C crk Proto-oncogene C-crk v crk avian sarcoma virus CT10 oncogene homolog v crk sarcoma virus CT10 oncogene homolog v crk sarcoma virus CT10 oncogene homolog (avian) |
Images
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Fig1:
Western blot analysis of Crk p38 on different lysates with Rabbit anti-Crk p38 antibody (HA722453) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: C6 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722453) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.