UBE2C Recombinant Rabbit Monoclonal Antibody [JE38-67]
cat.: HA722454
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE38-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human UBE2C aa 80-179 / 179. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, THP-1 cell lysate. |
| Subcellular location: | Cytosol. Nucleoplasm.Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O00762 Human |
| Alternative names: | Cyclin selective ubiquitin carrier protein dJ447F3.2 Mitotic specific ubiquitin conjugating enzyme UB E2C UBCH 10 UbcH10 UBE 2C Ube2c UBE2C_HUMAN Ubiquitin carrier protein C Ubiquitin carrier protein E2 C Ubiquitin carrier protein E2C Ubiquitin conjugating enzyme E2 C Ubiquitin conjugating enzyme E2C Ubiquitin protein ligase C Ubiquitin-conjugating enzyme E2 C Ubiquitin-protein ligase C |
Images
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Fig1:
Western blot analysis of UBE2C on different lysates with Rabbit anti-UBE2C antibody (HA722454) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722454) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.