ABO Recombinant Rabbit Monoclonal Antibody [JE39-25]
cat.: HA722455
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE39-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ABO aa 1-50 / 354. |
| Positive control: | HT-29 cell lysate, K-562 cell lysate, 293T cell lysate, rat skeletal muscle tissue lysate. |
| Subcellular location: | Golgi apparatus, Golgi stack membrane. Secreted. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P16442 Human |
| Alternative names: | A transferase Abo B transferase BGAT_HUMAN Blood Group H2 antigen CD173 Fucosylglycoprotein 3-alpha-galactosyltransferase Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form Glycoprotein-fucosylgalactoside alpha-galactosyltransferase Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase Histo-blood group A transferase Histo-blood group B transferase NAGAT |
Images
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Fig1:
Western blot analysis of ABO on different lysates with Rabbit anti-ABO antibody (HA722455) at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722455) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
ABO was immunoprecipitated from 0.2 mg 293T cell lysate with HA722455 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722455 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cell lysate (input) Lane 2: HA722455 IP in 293T cell lysate Lane 3: Rabbit IgG instead of HA722455 in 293T cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.