Phospho-PKC delta (Y311) Recombinant Rabbit Monoclonal Antibody [JE44-20]
cat.: HA722458
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE44-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Tyr311 of human PKC delta. |
| Positive control: | THP-1 cell lysate, THP-1 treated with 50ng/mL PMA for 72 hours cell lysate, THP-1 treated with 100ng/mL PMA for 72 hours cell lysate, mouse small intestine tissue lysate, rat lung tissue lysate. |
| Subcellular location: | Cell membrane. Cytoplasm. Membrane. Mitochondrion. Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q05655 Human | P28867 Mouse | P09215 Rat |
| Alternative names: | CVID9 D14Ertd420e Kinase PKC delta KPCD KPCD_HUMAN MAY 1 MAY1 MGC49908 nPKC delta nPKC-delta PCKd PKC d PKC delta PKCD PKCdelta PRKC D PRKC delta Prkcd Protein Kinase C delta Protein kinase C delta type Protein kinase C delta VIII Protein Kinase Cdelta Tyrosine protein kinase PRKCD |
Images
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Fig1:
Western blot analysis of Phospho-PKC delta (Y311) on different lysates with Rabbit anti-Phospho-PKC delta (Y311) antibody (HA722458) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 50ng/mL PMA for 72 hours cell lysate (20 µg/Lane) Lane 3: THP-1 treated with 100ng/mL PMA for 72 hours cell lysate (20 µg/Lane) Lane 4: Mouse small intestine tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Mouse small intestine tissue lysate, the membrane treated with λpp for 1 hour (40 µg/Lane) Lane 7: Rat lung tissue lysate, the membrane treated with λpp for 1 hour (40 µg/Lane) Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.