GSC Recombinant Rabbit Monoclonal Antibody [JE51-35]
cat.: HA722460
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE51-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GSC aa 1-50 / 257. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, BxPC-3 cell lysate, HeLa cell lysate, COS-1 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P56915 Human Entrez Gene: 317715 Rat |
| Alternative names: | Goosecoid goosecoid homeobox GSC GSC_HUMAN Homeobox protein goosecoid |
Images
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Fig1:
Western blot analysis of GSC on different lysates with Rabbit anti-GSC antibody (HA722460) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: BxPC-3 cell lysate Lane 4: HeLa cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722460) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.