CLP1 Recombinant Rabbit Monoclonal Antibody [JE61-01]
cat.: HA722462
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE61-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CLP1 aa 350-425. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, 293T cell lysate, Jurkat cell lysate, human colon tissue, mouse colon tissue, rat colon tissue, HeLa. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: Q92989 Human | Q99LI9 Mouse | Q5PQL4 Rat |
| Alternative names: | ATP/GTP binding protein Cleavage and polyadenylation factor I subunit 1 CLP 1 clp1 CLP1 cleavage and polyadenylation factor I subunit homolog CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) CLP1, yeast, homolog of CLP1_HUMAN hClp1 Homolog of yeast CFIA subunit Clp1p Polyadenylation factor Clp1 Polynucleotide kinase Clp1 Polyribonucleotide 5' hydroxyl kinase Clp1 Polyribonucleotide 5''-hydroxyl-kinase Clp1 Pre mRNA cleavage complex II protein Clp1 Pre-mRNA cleavage complex II protein Clp1 |
Images
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Fig1:
Western blot analysis of CLP1 on different lysates with Rabbit anti-CLP1 antibody (HA722462) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 1 minute 50 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722462) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CLP1 antibody (HA722462) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722462) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-CLP1 antibody (HA722462) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722462) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-CLP1 antibody (HA722462) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722462) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of HeLa cells labeling CLP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722462, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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