SCIN Recombinant Rabbit Monoclonal Antibody [JE62-89]
cat.: HA722465
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE62-89 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SCIN aa 616-715 / 715. |
| Positive control: | HEK-293 cell lysate, NIH/3T3 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9Y6U3 Human | Q60604 Mouse Entrez Gene: 298975 Rat |
| Alternative names: | Adseverin ADSV_HUMAN KIAA1905 Scin Scinderin |
Images
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Fig1:
Western blot analysis of SCIN on different lysates with Rabbit anti-SCIN antibody (HA722465) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722465) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.