PI3 Kinase p110 beta Recombinant Rabbit Monoclonal Antibody [JE65-38]
cat.: HA722474
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE65-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PI3 Kinase p110 beta aa 400-500. |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:200 1:50 |
| Uniprot #: | SwissProt: P42338 Human | Q3U4Q1 Mouse | Q8BTI9 Mouse | Q9Z1L0 Rat |
| Alternative names: | 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta 5-bisphosphate 3-kinase catalytic subunit beta isoform DKFZp779K1237 MGC133043 OTTHUMP00000216901 OTTHUMP00000216904 p110 BETA p110Beta Phosphatidylinositol 3 kinase catalytic beta polypeptide Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform Phosphatidylinositol-4 Phosphoinositide 3 kinase catalytic beta polypeptide PI3 kinase p110 subunit beta PI3-kinase subunit beta PI3K PI3K beta PI3K-beta PI3Kbeta PI3KCB PIK3C1 Pik3cb PK3CB_HUMAN PtdIns 3 kinase p110 PtdIns-3-kinase subunit beta PtdIns-3-kinase subunit p110-beta |
Images
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Fig1:
Western blot analysis of PI3 Kinase p110 beta on different lysates with Rabbit anti-PI3 Kinase p110 beta antibody (HA722474) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: Human brain tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 2 minutes 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722474) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PI3 Kinase p110 beta antibody (HA722474) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722474) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-PI3 Kinase p110 beta antibody (HA722474) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722474) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling PI3 Kinase p110 beta with Rabbit anti-PI3 Kinase p110 beta antibody (HA722474) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722474, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig5:
Immunofluorescence analysis of paraffin-embedded rat colon tissue labeling PI3 Kinase p110 beta with Rabbit anti-PI3 Kinase p110 beta antibody (HA722474) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722474, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.