ZW10 Recombinant Rabbit Monoclonal Antibody [PSH05-80]
cat.: HA722479
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH05-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 89 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ZW10 aa 350-779. |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, Ramos cell lysate, U-2 OS cell lysate, HCT 116 cell lysate, SW620 cell lysate, HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human testis tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Centromere, Chromosome, Cytoplasm, Cytoskeleton, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:2,000 |
| Uniprot #: | SwissProt: O43264 Human | O54692 Mouse | Q4V8C2 Rat |
| Alternative names: | Centromere/kinetochore protein zw10 Centromere/kinetochore protein zw10 homolog HZW 10 HZW10 Kinetochore associated homolog KNTC1AP MGC149821 Zeste white 10 Zeste white 10 homolog ZW 10 ZW 10 homolog centromere/kinetochore protein ZW 10 kinetochore associated homolog ZW10 (Drosophila) homolog centromere/kinetochore protein Zw10 ZW10 homolog centromere/kinetochore protein (Drosophila) ZW10 homolog centromere/kinetochore protein ZW10 kinetochore associated homolog ZW10, kinetochore associated, homolog (Drosophila) ZW10_HUMAN |
Images
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Fig1:
Western blot analysis of ZW10 on different lysates with Rabbit anti-ZW10 antibody (HA722479) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: Ramos cell lysate Lane 4: U-2 OS cell lysate Lane 5: HCT 116 cell lysate Lane 6: SW620 cell lysate Lane 7: HeLa cell lysate Lane 8: COS-1 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: C6 cell lysate Lane 11: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 89 kDa Observed band size: 89 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722479) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ZW10 antibody (HA722479) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722479) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-ZW10 antibody (HA722479) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722479) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ZW10 antibody (HA722479) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722479) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.