CD2 Recombinant Rabbit Monoclonal Antibody [PSH05-84]
cat.: HA722483
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH05-84 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD2 aa 25-209 (Extracellular). |
| Positive control: | Jurkat cell lysate, human T cell lymphoma tissue, human appendix tissue, human spleen tissue, human liver tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: P06729 Human |
| Alternative names: | CD 2 CD2 CD2 antigen (p50), sheep red blood cell receptor CD2 antigen CD2 molecule CD2_HUMAN Erythrocyte receptor FLJ46032 LFA-2 LFA-3 receptor LFA2 LFA3 receptor Ly-37 Lymphocyte function antigen 2 lymphocyte-function antigen-2 OTTHUMP00000024366 Rosette receptor Sheep erythrocyte receptor SRBC T cell surface antigen CD2 T-cell surface antigen CD2 T-cell surface antigen T11/Leu-5 T-lymphocyte surface CD2 antigen T11 |
Images
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Fig1:
Western blot analysis of CD2 on different lysates with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722483) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human T cell lymphoma tissue with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722483) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722483) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722483) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722483) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: Immunofluorescence (IF-tissue) Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA722483, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig7:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Primary antibody dilution: 1/1,000 Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes Platform: Leica Biosystems BOND® RX |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.