SESN2 / Sestrin-2 Recombinant Rabbit Monoclonal Antibody [PSH05-78]
cat.: HA722489
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH05-78 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SESN2 aa 231-280. |
| Positive control: | K-562 cell lysate, HEK-293 cell lysate, 293T cell lysate, HCT 116 cell lysate, LoVo cell lysate, HepG2 cell lysate, Jurkat cell lysate, U-87 MG cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P58004 Human | P58043 Mouse Entrez Gene: 502988 Rat |
| Alternative names: | DKFZp761M0212 DKFZp761M02121 Hi95 Hypoxia induced gene 95 MGC11758 OTTHUMP00000003641 OTTMUSP00000010184 RP23 63D8.3 SES2 SESN 2 SESN2 SESN2_HUMAN SEST2 Sestrin 2 Sestrin-2 |
Images
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Fig1:
Western blot analysis of SESN2 / Sestrin-2 on different lysates with Rabbit anti-SESN2 / Sestrin-2 antibody (HA722489) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: 293T cell lysate Lane 4: HCT 116 cell lysate Lane 5: LoVo cell lysate Lane 6: HepG2 cell lysate Lane 7: Jurkat cell lysate Lane 8: U-87 MG cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: RAW264.7 cell lysate Lane 11: C6 cell lysate Lane 12: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: Lane 1-8: 1 minute 18 seconds; Lane 9-12: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722489) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.