STIL / SIL Recombinant Rabbit Monoclonal Antibody [PSH05-79]
cat.: HA722490
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH05-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 143 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human STIL aa 351-900. |
| Positive control: | K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, 293T cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, cell cortex. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q15468 Human |
| Alternative names: | DKFZp686O09161 MCPH7 OTTHUMP00000009566 SCL interrupting locus protein SCL-interrupting locus protein SCL/TAL1 interrupting locus SIL STIL STIL_HUMAN TAL 1 interrupting locus protein TAL-1-interrupting locus protein |
Images
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Fig1:
Western blot analysis of STIL / SIL on different lysates with Rabbit anti-STIL / SIL antibody (HA722490) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 143 kDa Observed band size: 175 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722490) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.