Human CD276 Recombinant Rabbit Monoclonal Antibody [PSH05-93]
cat.: HA722492
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD276 aa 29-466 (HA210964). |
| Positive control: | Recombinant Human CD276 protein (HA210964). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-92] to Human CD276 (Capture) (HA722491) and Recombinant Human CD276 protein (HA210964) as the standard. The reference range value is 30.9-2500 pg/ml. |
| Uniprot #: | SwissProt: Q5ZPR3 Human |
| Alternative names: | 4Ig B7 H3 4Ig-B7-H3 AU016588 B7 H3 B7 homolog 3 B7-H3 B7H3 B7RP-2 CD_antigen=CD276 CD276 CD276 antigen CD276 molecule CD276_HUMAN Costimulatory molecule Flags: Precursor PSEC0249 UNQ309/PRO352 |
Images
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Fig1:
Sandwich ELISA analysis of Human CD276 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722491) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD276 protein (HA210964) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722491, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native B7-H3 in human serum and human urine samples. The concentrations of B7-H3 were measured in triplicates, interpolated from the B7-H3 standard curve and corrected for sample dilution. Undiluted samples are human serum 5% and human urine 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean B7-H3 concentration was determined to be 19.1ng/ml in human serum and 4.9 ng/ml in human urine. |
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Fig3:
Interpolated concentrations of native B7-H3 in A431 and NIH:OVCAR-3 cell culture supernatant. The concentrations of B7-H3 were measured in triplicates, interpolated from the B7-H3 standard curve and corrected for sample dilution. Undiluted samples are A431 cell culture supernatant 100% and NIH:OVCAR-3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean B7-H3 concentration was determined to be 199.5 pg/ml in A431 cell culture supernatant and 476.8 pg/ml in NIH:OVCAR-3 cell culture supernatant. |
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Fig4:
Interpolated concentrations of spiked B7-H3 in human cell culture media samples. The concentrations of B7-H3 were measured in triplicates, interpolated from the B7-H3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.