Human LIF Recombinant Rabbit Monoclonal Antibody [PSH05-97]
cat.: HA722496
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-97 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LIF aa 23-202 . |
| Positive control: | Recombinant standard Human LIF protein (HA210736). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-71] to Human LIF (Capture) (HA722423) and recombinant standard Human LIF protein (HA210736) as the standard. The reference range value is 8.2-2,000 pg/ml. |
| Uniprot #: | SwissProt: P15018 Human |
| Alternative names: | CDF Cholinergic Differentiation Factor D factor DIA Differentiation inducing factor differentiation inhibitory activity Differentiation stimulating factor Differentiation-stimulating factor Emfilermin Hepatocyte stimulating factor III HILDA Human interleukin in DA cells Leukemia inhibitory factor LIF LIF_HUMAN Melanoma derived LPL inhibitor Melanoma-derived LPL inhibitor MLPLI |
Images
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Fig1:
Sandwich ELISA analysis of human LIF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722495) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Human LIF protein (HA210736) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722496, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native LIF in cell culture supernatant and serum samples. LIF was measured in 100% cell supernatant. The concentrations of LIF were interpolated from the LIF standard curves. The mean LIF concentration was determined to be 148 pg/mL in MDA-MB-231 cell supernanant. There was no detectable signal in T-47D cell supernatant and human serum. |
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Fig3:
Interpolated concentrations of native LIF in human A375 cell culture supernatant samples. Interpolated concentration of native LIF was measured in duplicate at different sample concentrations. Undiluted samples were 12.5% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). |
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Fig4:
Interpolated concentrations of spiked LIF in human cell culture media samples. The concentrations of LIF were measured in duplicates, interpolated from the LIF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.