Human PDGF-BB Recombinant Rabbit Monoclonal Antibody [PSH05-99]
cat.: HA722498
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PDGF-B aa 82-190. |
| Positive control: | Recombinant standard Human PDGF-BB protein (HA210859). |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-98] to Human PDGF-BB (Capture) (HA722497) and recombinant standard Human PDGF-BB protein (HA210859) as the standard. The reference range value is 30.9-2500 pg/ml. |
| Uniprot #: | SwissProt: P01127 Human |
| Alternative names: | Becaplermin c sis FLJ12858 Oncogene SIS PDGF 2 PDGF B chain PDGF Beta PDGF subunit B PDGF-2 PDGF2 Pdgfb PDGFB_HUMAN Platelet derived growth factor 2 Platelet derived growth factor B chain Platelet derived growth factor beta Platelet derived growth factor beta polypeptide (oncogene SIS) Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) Platelet derived growth factor beta polypeptide Platelet-derived growth factor B chain Platelet-derived growth factor beta polypeptide Platelet-derived growth factor subunit B Proto-oncogene c-Sis Simian sarcoma viral (v sis) oncogene homolog SIS SSV v sis platelet derived growth factor beta polypeptide |
Images
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Fig1:
Sandwich ELISA analysis of Human PDGF-BB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722497) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant standard Human PDGF-BB Protein(HA210859) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722498, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native PDGF-BB in serum sample. Interpolated concentration of native PDGF-BB was measured in duplicate at different sample concentrations. Undiluted samples were 50% serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). |
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Fig3:
Interpolated concentrations of spiked PDGF-BB in human cell culture media samples. The concentrations of PDGF-BB were measured in duplicates, interpolated from the PDGF-BB standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.