TNFRSF14 Recombinant Rabbit Monoclonal Antibody [PSH06-02]
cat.: HA722501
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH06-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TNFRSF14 aa 1-223 / 283. |
| Positive control: | SK-MEL-28 cell lysate, SK-MEL-28. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:50 1:1,000 |
| Uniprot #: | SwissProt: Q92956 Human |
| Alternative names: | HVEML ATAR CD270 CD40 like protein precursor Herpes virus entry mediator A Herpesvirus entry mediator A Herpesvirus entry mediator Herpesvirus entry mediator ligand HveA HVEM HVEM L LIGHT LIGHTR TNFRSF14 TNFSF 14 TNR14_HUMAN TR2 Tumor necrosis factor receptor like gene2 Tumor necrosis factor receptor superfamily member 14 Tumor necrosis factor receptor superfamily member 14 precursor Tumor necrosis factor receptor-like 2 |
Images
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Fig1:
Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody (HA722501) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: SK-MEL-28 cell lysate treated with deglycosylation Lane 3: A549 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 40-50 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SK-MEL-28 (positive) and A549 (negative) labeling TNFRSF14 with Rabbit anti-TNFRSF14 antibody (HA722501) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TNFRSF14 antibody (HA722501) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of SK-MEL-28 (left, positive) and A549 (right, negative) cells labeling TNFRSF14. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722501, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.