MX1 Recombinant Rabbit Monoclonal Antibody [PSH06-03]
cat.: HA722502
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH06-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MX1 aa 351-662. |
| Positive control: | HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate, Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate, Daudi treated with 10ng/mL IFN-α1 for 16 hours, human spleen tissue. |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum membrane, Cytoplasm, perinuclear region; Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:50 1:200 |
| Uniprot #: | SwissProt: P20591 Human |
| Alternative names: | Human interferon regulated resistance GTP binding protein MXA IFI 78 IFI 78K IFI-78K IFI78 IFI78K Interferon induced GTP binding protein Mx1 Interferon induced protein p78 Interferon inducible protein p78 Interferon regulated resistance GTP binding protein Interferon regulated resistance GTP binding protein MxA Interferon-induced GTP-binding protein Mx1 Interferon-induced protein p78 Interferon-regulated resistance GTP-binding protein MxA MX 1 MX MX dynamin-like GTPase 1 MX1 MX1_HUMAN MxA Myxoma resistance protein 1 Myxovirus (influenza virus) resistance 1 interferon inducible Myxovirus (influenza virus) resistance 1 interferon inducible protein p78 Myxovirus (influenza) resistance 1 homolog of murine Myxovirus resistance 1, mouse, homolog of Myxovirus resistance protein 1 N-terminally processed |
Images
|
Fig1:
Western blot analysis of MX1 on different lysates with Rabbit anti-MX1 antibody (HA722502) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate Lane 3: Daudi cell lysate Lane 4: Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722502) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of Daudi cells treated with 10ng/mL IFN-α1 for 16 hours labeling MX1 with Rabbit anti-MX1 antibody (HA722502) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MX1 antibody (HA722502) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MX1 antibody (HA722502) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722502) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.