MAP3K1 Recombinant Rabbit Monoclonal Antibody [PSH06-10]
cat.: HA722507
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 165 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MAP3K1 aa 1413-1512. |
| Positive control: | A431 cell lysate, Jurkat cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, HeLa cell lysate, HL-60 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q13233 Human | P53349 Mouse | Q62925 Rat |
| Alternative names: | M3K1_HUMAN MAP3K1 MAPK/ERK kinase kinase 1 MAPKKK1 MEK kinase 1 MEKK 1 Mekk MEKK1 Mitogen activated protein kinase kinase kinase 1 Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase Mitogen-activated protein kinase kinase kinase 1 SRXY6 |
Images
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Fig1:
Western blot analysis of MAP3K1 on different lysates with Rabbit anti-MAP3K1 antibody (HA722507) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: HeLa cell lysate (20 µg/Lane) Lane 7: HL-60 cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: Mouse spleen tissue lysate (40 µg/Lane) Predicted band size: 165 kDa Observed band size: 130 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722507) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.