Pyrin Recombinant Rabbit Monoclonal Antibody [PSH06-12]
cat.: HA722509
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH06-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Pyrin aa 450-781. |
| Positive control: | THP-1 cell lysate, THP-1 treated with 1μg/mL LPS for 24 hours cell lysate, THP-1. |
| Subcellular location: | Cell projection. Cytoplasm. Cytoskeleton. Microtubule. Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: O15553 Human |
| Alternative names: | FMF Marenostrin Mediterranean fever Mediterranean fever protein MEF Mefv MEFV_HUMAN Pyrin TRIM20 |
Images
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Fig1:
Western blot analysis of Pyrin on different lysates with Rabbit anti-Pyrin antibody (HA722509) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 1μg/mL LPS for 24 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 86 kDa Observed band size: 86 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722509) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of THP-1 cells labeling Pyrin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722509, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.