Sortilin / NT3 Recombinant Rabbit Monoclonal Antibody [PSH06-16]
cat.: HA722513
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH06-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Sortilin aa 78-755 (Extracellular). |
| Positive control: | SW480 cell lysate, HeLa cell lysate, 293T cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, U-2 OS cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse lung tissue lysate, Rat brain tissue lysate, Rat lung tissue lysate, human testis tissue, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane. Endoplasmic reticulum. Endosome. Golgi apparatus. Lysosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q99523 Human | Q6PHU5 Mouse | O54861 Rat |
| Alternative names: | 100 kDa NT receptor Glycoprotein 95 Gp 95 Gp95 LDLCQ6 Neurotensin receptor 3 NT 3 NT3 NTR 3 NTR3 OTTHUMP00000013784 SORT 1 SORT_HUMAN SORT1 (gene name) Sort1 Sortilin 1 Sortilin |
Images
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Fig1:
Western blot analysis of Sortilin / NT3 on different lysates with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: SK-Br-3 cell lysate (20 µg/Lane) Lane 6: U-2 OS cell lysate (20 µg/Lane) Lane 7: U-87 MG cell lysate (20 µg/Lane) Lane 8: Neuro-2a cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: Mouse brain tissue lysate (40 µg/Lane) Lane 11: Mouse lung tissue lysate (40 µg/Lane) Lane 12: Rat brain tissue lysate (40 µg/Lane) Lane 13: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 100 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722513) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722513) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722513) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722513) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Sortilin / NT3 antibody (HA722513) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722513) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.