HA722514

YTHDF2 Recombinant Rabbit Monoclonal Antibody [PSH06-17]

cat.: HA722514

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Monkey
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	PSH06-17

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 62 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human YTHDF2 aa 519-579.
Positive control:	HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, HCT 116 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue, HCT 116.
Subcellular location:	Cytoplasm. Nucleus.
Recommended Dilutions: WB IHC-P FC	1:2,000 1:500 1:1,000
Uniprot #:	SwissProt: Q9Y5A9 Human \| Q91YT7 Mouse Entrez Gene: 313053 Rat
Alternative names:	9430020E02Rik CAHL CLL associated antigen KW 14 CLL-associated antigen KW-14 HGRG8 High glucose regulated protein 8 High-glucose-regulated protein 8 NY REN 2 Renal carcinoma antigen NY REN 2 Renal carcinoma antigen NY-REN-2 YTH domain family 2 YTH domain family member 2 YTH domain family protein 2 YTH N6 methyladenosine RNA binding protein 2 YTHD2_HUMAN YTHDF 2 YTHDF2

Images

Fig1: Western blot analysis of YTHDF2 on different lysates with Rabbit anti-YTHDF2 antibody (HA722514) at 1/2,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: MDA-MB-231 cell lysate
Lane 6: COS-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722514) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-YTHDF2 antibody (HA722514) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722514) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-YTHDF2 antibody (HA722514) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722514) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-YTHDF2 antibody (HA722514) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722514) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-YTHDF2 antibody (HA722514) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722514) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-YTHDF2 antibody (HA722514) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722514) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Flow cytometric analysis of HCT 116 cells labeling YTHDF2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722514, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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