LSD2 / AOF1 Recombinant Rabbit Monoclonal Antibody [PSH06-18]
cat.: HA722515
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LSD2 aa 1-450. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, HCT 116 cell lysate, K-562 cell lysate, A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate. |
| Subcellular location: | Chromosome. Nucleus. |
| Recommended Dilutions:
WB |
1: 2,000 |
| Uniprot #: | SwissProt: Q8NB78 Human | Q8CIG3 Mouse Entrez Gene: 306819 Rat |
| Alternative names: | amine oxidase (flavin containing) domain 1 amine oxidase, flavin containing 1 AOF1 bA204B7.3 C6orf193 dJ298J15.2 Flavin-containing amine oxidase domain-containing protein 1 FLJ33898 FLJ34109 FLJ43328 KDM1B KDM1B_HUMAN LSD2 lysine (K)-specific demethylase 1B Lysine-specific demethylase 2 Lysine-specific histone demethylase 1B Lysine-specific histone demethylase 2 |
Images
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Fig1:
Western blot analysis of LSD2 / AOF1 on different lysates with Rabbit anti-LSD2 / AOF1 antibody (HA722515) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HCT 116 cell lysate Lane 4: K-562 cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: RAW264.7 cell lysate Lane 9: Neuro-2a cell lysate Lane 10: PC-12 cell lysate Lane 11: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722515) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.